Kick off: 20:30 June 23 - Australia need to keep 11 players on the pitch at Nelspruit if they are to have any hope of qualifying for the last 16
In both matches so far at South Africa 2010 the Socceroos have had a man sent off, key midfielder Tim Cahill seeing red in the 4-0 drubbing by Germany and striker Harry Kewell in the 1-1 draw with Ghana.
Cahill is available again on Wednesday, but Kewell is suspended - as is defender Craig Moore, who has picked up two bookings.
"We know we haven't got the rub of the green, which is disappointing," said midfield player Brett Emerton. "We've played the majority of this World Cup with 10 men so far and both have been very, very harsh red cards.
"It's disappointing to lose Harry, but we get Tim back and after our good performance against Ghana, we should go into this match full of confidence."
The point against the Africans was the Socceroos' first of the tournament, which has left Pim Verbeek's squad bottom of Group D, but if they beat Serbia and Ghana defeat Germany, it will all come down to goal difference.
However, Emerton stressed that Australia would not be employing all-out attack from the whistle against the Serbs.
"We won't go gung-ho," he said. "We will still have a strategic plan and obviously we'll be looking to win the game and hopefully Ghana can come up with an upset against Germany.
"I've done the calculations plenty of times. We've all talked about the different scenarios. It's still possible. Germany could beat Ghana by three or four and that means we can beat the Serbs by one or two. If we get the win, we can walk away with our heads held high."
With Australia still in with a chance of making the last 16, Serbia recognise they must come back down to earth after their shock 1-0 victory over Germany.
Goalkeeper Vladimir Stojkovic, who saved a penalty in that match, said: "I'm overjoyed, because we desperately needed the win, but we're focusing completely on our last game now, because it won't be easy."
Serbia coach Radomir Antic has to decide which - if any - of the six players who are on one yellow card to leave out.